精品文档---下载后可任意编辑UBA52 与 Hsf4b 的相互作用及调控的开题报告摘要：UBA52 是一种结合核糖体蛋白复合物的蛋白质，并参加蛋白降解和特异性翻译后修饰。Hsf4b 是一种基因转录因子，参加眼球透明质的生长和维护。本文旨在探究 UBA52 与 Hsf4b 之间的相互作用及其调控机制。首先，通过蛋白质相互作用分析验证了 UBA52 与 Hsf4b 之间的物理相互作用；其次，运用免疫印迹法、蛋白质稳定性分析和荧光共聚焦显微镜技术，发现 Hsf4b 通过促进 UBA52 的泛素化和降解来调控UBA52 的表达水平和功能；最后，通过小鼠模型验证了 UBA52 与Hsf4b 相互作用对眼球透明度和复视能力的影响。关键词：UBA52、Hsf4b、相互作用、调控、眼球透明质Abstract：UBA52 is a protein that binds to the ribosomal protein complex and is involved in protein degradation and specific translator post-modification. Hsf4b is a gene transcription factor involved in the growth and maintenance of eye transparency. This article aims to explore the interaction between UBA52 and Hsf4b and its regulatory mechanism. Firstly, the physical interaction between UBA52 and Hsf4b was verified by protein interaction analysis. Secondly, through immunoblotting, protein stability analysis and fluorescence co-localization microscopy technology, it was found that Hsf4b regulates the expression and function of UBA52 by promoting UBA52 ubiquitinating and degradation. Finally, a mouse model was used to verify the effect of the interaction between UBA52 and Hsf4b on eye transparency and visual fatigue.Keywords: UBA52, Hsf4b, interaction, regulation, eye transparency.
