常用蛋白酶切割位点(3 页)Good is good, but better carries it.精益求精，善益求善。常用融合蛋白切割位点PROTEASE COMPANY Protease Capture Thrombin Leu-Val-Pro-Arg▼Gly-SerL-V-P-R▼G-SAmersham-Biosciences, Novagen, SIGMA, RocheBenzamidine-AgaroseFactor Xa Ile-Glu/Asp-Gly-Arg▼ I-E/D-G-R▼Amersham-Biosciences, New England Biolabs,RocheBenzamidine-AgaroseEnterokinase Asp-Asp-Asp-Asp-Lys▼D-D-D-D-K▼New England Biolabs, Novagen, RocheTrypsin Inhibitor-AgaroseTEV protease Glu-Asn-Leu-Tyr-Phe-Gln▼GlyE-N-L-Y–F-Q▼GInvitrogen – Life Technologies Ni-NTA (6His recomb. TEV)PreScission Leu-Glu-Val-Leu-Phe-Gln▼Gly-ProL-E-V-L-F-Q▼G-PAmersham-BiosciencesGSTrap for GST fusion enzymeTAGZyme His-tag removal by Exoproteolytic DigestionQiagenNi-NTA (6His recomb. enzyme)Intein Site dithiothreitol cleavageNew England BiolabsDTT elimination by dialysis HRV 3C Protease Leu-Glu-Val-Leu-Phe-Gln▼Gly-ProNovagen Ni-NTA (6His recomb. enzyme)L-E-V-L-F-Q▼G-PSUMO Protease recognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO Invitrogen – Life Technologies, LifeSensorsNi-NTA (6His recomb. enzyme)Kex-2-Arg-X-Lys/Arg-Arg▼Invitrogen – Life Technologies,Ni-NTA (6His recomb. enzyme)KEX2 对 arg 的专一性高，要求最重要。Arg 前为 lys 效率最高，不切-Arg-lys，Pro 影响 KEX2 切割羧肽酶羧肽酶 B 可以切割 C 端的 Lys 或Arg；羧肽酶 A 可以切割 C 端除了Lys、Arg、Pro 的氨基酸，但假如倒数第二个氨基酸为 Pro 两种羧肽酶均不能作用1.胰蛋白酶属肽链内切酶，能把多肽链中 Lys 和 Arg 残基中的羧基侧切断。2.胰凝乳蛋白酶（亦称糜蛋白酶）属肽链内切酶，主要切断多肽链中的芳香族氨基酸（Phe、Trp、Tyr）残基的羧基一侧。3.羧肽酶（分 A 和 B 型），一般的题目中没有特别指明的话就是两种类型的功能都具备，可以从羧基端切除氨基酸（若羧基端的第 1 个或第 2 个氨基酸为Pro 的则不能切除）。4.溴化氰处理，专一性的切割甲硫氨酸羧基端的肽键。}{@【《（~~~%?/（-"&~[？"…"@~"）"@'>
