AcceptedManuscriptComparisonofCCD-equippedlaserspeckleflowgraphywithhydrogengasclearancemethodinthemeasurementofopticnerveheadmicrocirculationinrabbitsHiroakiTakahashi,TetsuyaSugiyama,HidekiTokushige,TakatoshiMaeno,ToruNakazawa,TsunehikoIkeda,MakotoAraiePII:S0014-4835(12)00365-XDOI:10.1016/j.exer.2012.12.003Reference:YEXER6072Toappearin:ExperimentalEyeResearchReceivedDate:6October2012RevisedDate:9December2012AcceptedDate:10December2012Pleasecitethisarticleas:Takahashi,H.,Sugiyama,T.,Tokushige,H.,Maeno,T.,Nakazawa,T.,Ikeda,T.,Araie,M.,ComparisonofCCD-equippedlaserspeckleflowgraphywithhydrogengasclearancemethodinthemeasurementofopticnerveheadmicrocirculationinrabbits,ExperimentalEyeResearch(2013),doi:10.1016/j.exer.2012.12.003.ThisisaPDFfileofanuneditedmanuscriptthathasbeenacceptedforpublication.Asaservicetoourcustomersweareprovidingthisearlyversionofthemanuscript.Themanuscriptwillundergocopyediting,typesetting,andreviewoftheresultingproofbeforeitispublishedinitsfinalform.Pleasenotethatduringtheproductionprocesserrorsmaybediscoveredwhichcouldaffectthecontent,andalllegaldisclaimersthatapplytothejournalpertain.MANUSCRIPTACCEPTEDACCEPTEDMANUSCRIPT1/24Title:ComparisonofCCD-equippedlaserspeckleflowgraphywithhydrogengas1clearancemethodinthemeasurementofopticnerveheadmicrocirculationinrabbits23Authornamesandaffiliations:4HiroakiTakahashia,TetsuyaSugiyamab,HidekiTokushigea,TakatoshiMaenoc,Toru5Nakazawad,TsunehikoIkedab,MakotoAraiee67aResearchLaboratoryforDrugDevelopment,SenjuPharmaceuticalCo.,Ltd.,1-5-4,8Murotani,Nishi-ku,Kobe,Hyogo651-2241,Japan9bDepartmentofOphthalmology,OsakaMedicalCollege,2-7Daigaku-machi,Takatsuki,10Osaka569-8686,Japan11cDepartmentofOphthalmology,TohoUniversitySakuraMedicalCenter,564-112Shimoshizu,Sakura,Chiba285-8741,Japan13dDepartmentofOphthalmology,TohokuUniversityGraduateSchoolofMedicine,1-114Seiryo-machi,Aoba-ku,Sendai,Miyagi980-8574,Japan15eKantoCentralHospitalofTheMutualAidAssociationofPublicSchoolTeachers,166-25-1Kamiyoga,Setagaya-ku,Tokyo158-0098,Japan1718MANUSCRIPTACCEPTEDACCEPTEDMANUSCRIPT2/24Correspondingauthor19Tel.:+81726831221;fax:+81726818195.20E-mailaddress:tsugiyama@poh.osaka-med.ac.jp(T.Sugiyama).2122Abstract23Theaimofthisstudywastoverifythecorrelationbetweenmeanblurrate24(MBR)obtainedwithCCD-equippedlaserspeckleflowgraphy(LSFG)andcapillary25bloodflow(CBF)obtainedbythehydrogengasclearancemethodinrabbitopticnerve26head(ONH).UsingJapanesewhiterabbitsundersystemicanesthesia,ahydrogen27electrodewasinsertedanareaoftheONHfreefromsuperficialcapillaries.MBRwas28measuredwithLSFGnearthehydrogenelectrode.CBFandMBRweremeasuredinthe29rangeof32.4-83.5mL/min/100gand3.5-6.0,respectively.AMBRandCBFwere30significantlycorrelated(r=0.73,P<0.01,n=14).Afterinhalationofcarbondioxide31(CO2)orintravenousadministrationofendothelin-1(ET-1),MBRandCBFwere32changedintherelativerangeof0.74-1.27and0.76-1.35,respectively.Therelative33changesinMBRandCBFinducedbyCO2andET-1werealsosignificantlycorrelated34(r=0.67,P<0.01).ThecurrentresultssuggestthatMBRmaycorrelatewithCBFand35alsochangewithCBF,asanindexofbloodflowintheONH,linearly.36MANUSCRIPTACCEPTEDACCEPTEDMANUSCRIPT3/2437Keywords:38Opticnervehead;laserspeckleflowgraphy;meanblurrate;hydrogengasclearance39method;capillarybloodflow;rabbit4041Abbreviations42CBF:capillarybloodflow;LSFG:laserspeckleflowgraphy;MBR:meanblurrate;43...
