表122种磺胺标准品Table1Thestandardcompoundsof22sulfonamides结构式StructureNR1HSNOR2HO磺胺名称SulfonamidesR1R2CASpKa分子式Formula分子量MW磺胺胍SulfaguanidineHHCNHNH257-67-011.3C7H10N4O2S214.24磺胺SulfanilamideHH63-74-110.4C6H8N2O2S172.20磺胺嘧啶SulfadiazineHNN68-35-96.5C10H10N4O2S250.28磺胺二甲异嘧啶SulfisomidineHNNCH3CH3515-64-07.4C12H14N4O2S278.33磺胺醋酰SulfacetamideHHCOCH3144-80-95.4C8H10N2O3S214.24磺胺噻唑SulfathiazoleHNS72-14-07.2C9H9N3O2S2255.32磺胺吡啶SulfapyridineHN144-83-28.4C11H11N3O2S249.29磺胺甲基嘧啶SulfamerazineHNNCH3127-79-77.0C11H12N4O2S264.30磺胺对甲氧嘧啶SulfameterHNNOCH3651-06-96.7C11H12N4O3S280.30磺胺二甲噁唑SulfamoxoleHNOCH3CH3729-99-77.2C11H13N3O3S267.30磺胺二甲嘧啶SulfamethazineHNNCH3CH357-68-17.4C12H14N4O2S278.33磺胺甲噻二唑SulfamethizoleHNNSCH3144-82-15.5C9H10N4O2S2270.33磺胺甲氧哒嗪SulfamethoxypyridazineHNNOCH380-35-36.7C11H12N4O3S280.30琥珀酰磺胺噻唑SuccinylsulfathiazoleCHOH2CH2CCOOHNS116-43-8-C13H13N3O5S2355.39磺胺氯哒嗪SulfachloropyridazineHNNCl80-32-05.9C10H9ClN4O2S284.72磺胺甲基异噁唑SulfamethoxazoleHNOCH3723-46-66.0C10H11N3O3S253.28磺胺间甲氧嘧啶SulfamonomethoxineHNNOCH31220-83-36.5C11H12N4O3S280.30磺胺邻二甲氧嘧啶SulfadoxineHNNOCH3H3CO2447-57-65.9C12H14N4O4S310.33磺胺二甲异噁唑SulfisoxazoleHONCH3H3C127-69-55.0C11H13N3O3S267.30磺胺间二甲氧嘧啶SulfadimethoxineHNNOCH3OCH3122-11-26.3C12H14N4O4S310.33磺胺喹噁啉SulfaquinoxalineHNN59-40-55.5C14H12N4O2S300.34磺胺硝苯SulfanitranCHH3CONO2122-16-714.5C14H13N3O5S335.342.2色谱条件WatersACQUITYUPLCBEHC18柱（1.7µm,2.1mm×50mm）；流动相：甲醇（A）和0.1%甲酸水溶液（B），梯度洗脱程序：0~2.6min，5%A~16%A；2.6~3.8min，16%A~22%A；3.8~4.8min，22%A~25%A；4.8~6.6min，25%A；6.6~7.0min，25%A~55%A；7.0~9.2min，55%A~95%A；9.2~10.0min，95%A，10.0~11.0min，95%A~8%A；流速0.2mL/min；柱温30℃；样品室温度20℃；进样量5µL。2.3质谱条件电喷雾离子源；正离子扫描；毛细管电压3.5kV；射频透镜电压0.0V；离子源温度120℃；去溶剂气温度350℃；去溶剂气流量500L/h；锥孔气流量50L/h；光电倍增器电压650V；碰撞气体为氩气，碰撞气压3.2×10-3mbar；多反应监测（MRM）模式检测，22种磺胺的质谱分析参数见表2。表222种磺胺的质谱分析参数Table2MSparametersfor22sulfonamidesanalysis磺胺名称Sulfonamides保留时间Retentiontime(min)监测离子对Monitoringionpairs(m/z)锥孔电压Conevoltage(V)碰撞能量CollisionEnergy(eV)215.8/108.62223磺胺胍Sulfaguanidine0.89215.8/156.92215173.2/91.41518磺胺Sulfanilamide0.99173.2/155.9157215.2/91.53020磺胺嘧啶Sulfadiazine1.84215.2/156.03017251.1/107.72523磺胺二甲异嘧啶Sulfisomidine2.43251.1/156.02515279.2/123.81522磺胺醋酰Sulfacetamide2.67279.2/186.11510256.2/107.62523磺胺噻唑Sulfathiazole3.00256.2/156.02515250.2/156.02715磺胺吡啶Sulfapyridine3.22250.2/184.22717265.2/156.02717磺胺甲基嘧啶Sulfamerazine3.52265.2/172.02715281.2/156.02518磺胺对甲氧嘧啶Sulfameter4.44281.2/215.22518268.2/112.72323磺胺二甲噁唑Sulfamoxole4.52268.2/156.02315279.2/156.02517磺胺二甲嘧啶Sulfamethazine4.59279.2/186.12515271.2/107.72617磺胺甲噻二唑Sulfamethizole4.70271.2/156.02617281.2/107.72727磺胺甲氧哒嗪Sulfamethoxypyridazine5.05281.2/156.02717琥珀酰磺胺噻唑5.32356.1/156.13223Succinylsulfathiazole356.1/256.23217285.2/107.72525磺胺氯哒嗪Sulfachloropyridazine5.44285.2/156.02515254.2/107.72522磺胺甲基异噁唑Sulfamethoxazole5.78254.2/156.02517281.1/156.02817磺胺间甲氧嘧啶Sulfamonomethoxine5.89281.1/215.22815311.2/107.73030磺胺邻二甲氧嘧啶Sulfadoxine6.21311.2/156.03018268.2/112.72315磺胺二甲异噁唑Sulfisoxazole6.50268.2/156.02313311.2/107.82525磺胺间二甲氧嘧啶Sulfadimethoxine8.05311.2/156.12515301.2/107.83527磺胺喹噁啉Sulfaquinoxaline8.12301.2/156.13520336.2/156.12513磺胺硝苯Sulfanitran8.49336.2/294.32510
