0DDDDDDDDDDDDDDDDDDDD2012D5DDDDDDDDDDDDDDDDDDDDDDDDDDD3.DDDDDDDDDDDDDDDDDDDDDDDDD4DDDDDDNADDDDDDDDDDDDDDDDDD4DDDDDDDDDDDDDDDDDDDDDDDDD6DDDDDDDDDDDDDDDDDDDDDDDDD10DDDDDDDDDDDDDDDDDDDDDDDDD12DDDDDDDDDDDDDDDDDDDDDDDDDD12DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDGeneticallyModifiedOrganismUMDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDglyphosateDDDDDDDDroundupreadysoybeanUMMDDDVarietyA5403DDDDDDDCAMV—35SDDDDDDDDDDCP4—EPSPSUNOSDDDDDDDDDDDDDDDDDDDDDDDDDDDDD800DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDPCRDDDDDPCRDDDDDDDDDDDDPCRDDDPCRDDDDDPCRDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD2003DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDSN/T1195—2003皿叮(SN/T1196-2003)DDD(SN/T1199—2003)DDDD(SN/T1197—2003)DDD(SN/T1200—2003)DDDD(SN/T1198—2003)DDDDDDDDDDDDDDDDDDDDDDDDD2004DDDDDDDDDDDDDDD(GB/T19495.4—2004)0DDDDDDDDDDDDPCRDDDDDDPCRDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDNADDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDPCRDDDDDDDDDDDDDDDDDDDDDDDDDDDDDCAMV-35SDDDDDDDDDDDDEPSPSDDDDDDDODDDDDDDDD□□□□□□□□□□□□□□□□□11200叮1D□□□□□□□□□□□□□□□□IRMM—410RUFluka叮口2D□□□□□□□□□3D□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□1D□□□□□□□□□□□□□□□□□□□□4叮2D□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□3D□□□□□4D□□5D□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□1/16U6D□□□□□□□□□□ODDDDDNADDDDDDDCTAB叮DDDDDODDCTABDDDDDDDDDNADDDDDDDDDDDDDDDDD1DCTAB(hexadecyltrimethylammoniumbromide,DDDDDDDDDD)DDDDDDDODD,DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD(>0.7mol/LNaCI),CTABDDDDDDDDDDDDDDDDDDDDDD.DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDCTABDDDDD15DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD15DD2DCTABDDDDDDDDDDDDD1DTris-HCl(pH8.0)DDDDDDDD,DDDDDDDD2DEDTADDMg2+DMn2+DD，叮DNaseDDD3DNaClDDDDDDDD，口DNPDDDD,DDDDDDD4DCD-DDDDDDDDD,DDDDDDDDDD,DDDD,DDDDDDDDDDNAD5DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDIRMM-DDDDDDDDDDDDDDDD1DDDDDD75%DDDD3min,DDDDD3~2IgDDDDDDDDDDDDDDDDD2mlDDD3600卩65DDD30min.4500ulDDDDDDDD25:2412000r/minDDD15minD5DDDDDDDDDDDDDDDDD12000r/minDDD10minD6DDDDD70%DDDD120007DDDDDD50卩85卩37D30mi9400卩1250卩12000r/minDDD15minD1DDDDDDDDDDDD200卩12000r/minDDD10minD1DDDDDDTEDDDDDDDDDDDNDDDD1.DDDDDDDNADD,DDDDDDDD,DD,DDDDDDDDD-2.DDDDDDDDDDNADDDDDDDDDDDDDDODDDDDDNADDDDNADDDDDDDDDDDDODDDDDDDDDD1.DDDDDDODDEPDODDDD2.DD:DDDDDDDD-ODDDDCTABODDDCTAB20g/LDTris-HCI0.1mol/L(pH8.0)DEDTA0.02mol/LTEDDDDlommol/LTris.D1mmol/I,EDTADpH8.0).5卩g/卩l.DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDNA□□□□□□□□□□□□118bpU叮Lectin叮叮口DDDDDDDDDDDDDDDDDlectinDDD□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□DNA□□□□□□□□□2叮口□□PCR□□□□□□□□□□□□□□□3叮叮口□□□□□□0.1ng叮口DNAD4叮口□□□□□□GB/T19495.1U2004□□□□□□□10XPCR□□□□□□□□□□□□□□□□25mmol/L.dNTP□□□□2.5mmol/L.□□□A叮叮口□□Lectin□□□GeneBank®□□□K00821D5D-GCCCTCTACTCCACCCCCATCC-3DBDDDDD□□Lectin□叮GeneBank®□□□K00821D5D-GCCCATCTGCAAGCCTTTTTGTG-3DTaq□□5IU/UL5叮叮PCR叮□□□6DPCR叮PCR□□□□□□□□□□PCR□□□□□□□25DL叮叮叮口□□□□□□□□□□□□□□□□a□(A?)B.1PCR□□□□□□□□□□□□□/□L□□DNA10ng~50ng1□15.910xPCR叮口□□□□□□□1x2.5□□□□□□25mmol/LQ1.5mmol/L1.5dNTP叮口10mmol/LQ0.8mmol/L2DDDDDDDDDD□takaraDDD叮-DDDDDD-DNAExtractionKitforGMODetectionDP...
