精品文档---下载后可任意编辑候选基因集法及 IGF1-FoxO 通路与猪生长发育的关联讨论的开题报告摘要候选基因集法是利用已知的基因信息作为基础，通过比较不同生理状态或表型的个体，筛选出可能与该特定生理过程相关的基因集，进而进行深化讨论。本讨论将运用候选基因集法，探讨 IGF1-FoxO 通路与猪生长发育的相关性。讨论将使用猪为讨论对象，选取生长发育过程中的关键时期，进行基因表达谱分析，筛选出与生长发育相关的基因集。进一步，使用基因编辑技术构建基因敲除猪模型，验证筛选得到的候选基因集的功能。讨论结果有望为猪生长发育调控机制提供新的理论支持。关键词：候选基因集法、IGF1-FoxO 通路、猪、生长发育、基因编辑技术AbstractThe candidate gene set approach is a method that uses known gene information as a basis to select gene sets that may be related to specific physiological processes by comparing individuals under different physiological or phenotypic states, and conducting in-depth research on these gene sets. In this study, the candidate gene set approach will be used to explore the correlation between the IGF1-FoxO pathway and pig growth and development. Pigs will be used as the study objects, and key periods of growth and development will be selected for gene expression profiling analysis to screen for gene sets related to growth and development. Furthermore, gene-editing technology will be used to construct gene knockout pig models to verify the functions of the selected candidate gene sets. The results of this study are expected to provide new theoretical support for regulating the growth and development of pigs.Keywords: candidate gene set approach, IGF1-FoxO pathway, pig, growth and development, gene editing technology.
