渗透压敏感和耐高渗酵母菌在海藻酸钠-壳聚糖-海藻酸钠微囊中的生长和代谢VIP专享VIP免费

生物工程学报ChinJBiotech2008,July25;24(7):1274-1278journals.im.ac.cnChineseJournalofBiotechnologyISSN1000-3061cjb@im.ac.cn©2008InstituteofMicrobiology,CAS&CSM,AllrightsreservedReceived:October29,2007;Accepted:January16,2008Supportedby:theNationalNaturalScienceFoundationofChina(No.20736006),“Eleventh·Five”NationalKeyTechnologyR&DProgram(No.2006BAD27B04),theNationalBasicResearchProgramofChina(No.2007CB714305),andtheYouthTalentScienceFoundationofDalianCity(No.2007J23JH036).Correspondingauthor:XiaojunMa.Tel:+86-411-84379139;Fax:+86-411-84379139;E-mail:maxj@dicp.ac.cn国家自然科学重点基金(No.20736006),“十一五”国家科技支撑计划项目(No.2006BAD27B04),国家科技部“973”项目(No.2007CB714305),大连市青年科技人才基金(No.2007J23JH036)资助。研究简报渗透压敏感和耐高渗酵母菌在海藻酸钠-壳聚糖-海藻酸钠微囊中的生长和代谢宁小娟1,2,孙志杰1,钟盛华2,林军章1,吕国军1,于炜婷1,朱静1,王为1,马小军11中国科学院大连化学物理研究所生物医学材料工程组,大连1160232江西农业大学理学院,南昌330045摘要:为了研究微囊微环境中渗透压对微囊内不同渗透压敏感性细胞生长、代谢的影响,分别以渗透压敏感型酿酒酵母Y02724与耐高渗酵母Hansel为细胞模型,考察了有氧条件下这两种细胞在海藻酸钠-壳聚糖-海藻酸(Alginate-chitosan-alginate,ACA)微胶囊中的生长、代谢状态。主要检测了细胞比生长速率、最大产物生成量以及代谢物乙醇、甘油分泌量等的变化。实验结果分析表明,渗透压胁迫可能是导致不同渗透压敏感性细胞在微囊微环境中生长代谢特征变化的因素之一,即微囊微环境内可能存在渗透压胁迫。关键词:酵母,渗透压胁迫,ACA微囊,微囊微环境,生长代谢GrowthandMetabolismofOsmo-sensentiveYeastY02724andHigh-osmoticResistantYeastHanselinAlginate-chitosan-alginateMicrocapsulesXiaojuanNing1,2,ZhijieSun1,ShenghuaZhong2,JunzhangLin1,GuojunLü1,WeitingYu1,JingZhu1,WeiWang1,andXiaojunMa11DalianInstituteofChemicalPhysics,ChineseAcademyofSciences,Dalian116023,China2CollegeofScience,JiangxiAgriculturalUniversity,Nanchang330045,ChinaAbstract:Tostudytheeffectoftheosmoticstressinthemicroenvironmentonthegrowthandmetabolismoftheencapsulatedcellsunderaerobiccondition,Osmo-sensitiveyeastY02724andhigh-osmoticresistantyeastHanselwereusedasmodelstoexplorethegrowthandmetabolismstateofthecellscultivatedinalginate-chitosan-alginate(ACA)microcapsules.Thechangesoftheyeastcells’specificgrowthrate,maximumproductquantityandthesecretionofethanolandglycerolwereanalyzed.ForY02724,theyieldofethanolwasincreasedintheACAmicroenvironmentcomparedtosuspensioncultivation.ForHansel,themaximumgrowthspeedofmicroencapsulatedcultivationhadnoobviousdifferencecomparedtothesuspensioncultivation.Moreover,afterencapsulation,theproductionofglycerolwasdecreasedforbothY02724andHanselcomparedtosuspensioncultivation.Inconclusion,osmoticstressexistedintheACAmicrocapsulesandaffectedthegrowthandmetabolismofthecells.Keywords:yeast,osmoticstress,alginate-chitosan-alginatemicrocapsule,microenvironment,growthandmetabolism宁小娟等:渗透压敏感和耐高渗酵母菌在海藻酸钠-壳聚糖-海藻酸钠微囊中的生长和代谢1275Journals.im.ac.cn随着对生物技术和膜技术研究及认识的不断深入,微胶囊的应用领域正在不断拓宽,应用前景非常广阔。作为一种极有前途的载体,微胶囊已被用于动物细胞培养、细胞和酶的固定化、生化药物控制释放、抗癌药物筛选、人工器官及基因运载工具等生物医学领域[1,2]。海藻酸盐、壳聚糖等天然多糖资源丰富、制备简单、价格便宜,极具作为生物微胶囊制备材料的潜力。将天然材料与合成高分子混合作为一种材料,既利用合成材料弥补天然材料强度不足,又利于弥补合成材料生物相...