电脑桌面
添加小米粒文库到电脑桌面
安装后可以在桌面快捷访问

抗VEGF抗体及抗KDR抗体对裸鼠胃癌细胞增殖和凋亡的影响

抗VEGF抗体及抗KDR抗体对裸鼠胃癌细胞增殖和凋亡的影响_第1页
1/26
抗VEGF抗体及抗KDR抗体对裸鼠胃癌细胞增殖和凋亡的影响_第2页
2/26
抗VEGF抗体及抗KDR抗体对裸鼠胃癌细胞增殖和凋亡的影响_第3页
3/26
抗 VEGF 抗体及抗 KDR 抗体对裸鼠胃癌细胞增殖和凋亡的影响 【关键词】 抗体 The Effect of Anti vascular Endothelial Growth Factor and Receptor Antibody on the Proliferation Activity and Apoptosis of Human Gastric Cancer Abstract:Objective To study the inhibiting effect of anti vascular endothelial growth factor and receptor antibody on growth、metastasis and apoptosis of gastric cancer. Methods The inhibitory effect of anti VEGF antibody, anti KDR antibody were investigated by means of an orthotopic xenotransplanted model of mice which had been randomly divided into 4 groups: ①control group, ②group receiving anti VEGF antibody, ③group receiving anti KDR antibody and ④group receiving anti VEGF antibody and anti KDR antibody. Anti VEGF antibody and anti KDR antibody was given twice a week for 8 weeks from day 7 after animals were sacrificed at the end of 10 weeks. The tumor was weighted and the expression level of VEGF mRNA and protein were examined in antisense group by RT PCR analysis and immunohistochemistry respectively. Apoptotic cell and cell cycle were detected in transfected cells by FCM assay. Results The decreased expression levels of VEGF protein were found in anti VEGF antibody group,anti KDR antibody group, anti VEGF antibody and anti KDR antibody group. Apoptotic cells were found in three groups’cells. G0/G1 phase cells were increased and S phase cells were decreased in cell cycle. Conclusions Both blockadiage of VEGF with anti VEGF antibody and VEGF binding site with anti KDR antibody could inhibited gastric cancer. cells growth,which might provide a new approach to the treatment of gastric cancer. Key words:Gastric cancer;Vascular endothelial growth factor;Rec...

1、当您付费下载文档后,您只拥有了使用权限,并不意味着购买了版权,文档只能用于自身使用,不得用于其他商业用途(如 [转卖]进行直接盈利或[编辑后售卖]进行间接盈利)。
2、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。
3、如文档内容存在违规,或者侵犯商业秘密、侵犯著作权等,请点击“违规举报”。

碎片内容

抗VEGF抗体及抗KDR抗体对裸鼠胃癌细胞增殖和凋亡的影响

确认删除?
VIP
微信客服
  • 扫码咨询
会员Q群
  • 会员专属群点击这里加入QQ群
客服邮箱
回到顶部