缓冲液配制方法VIP免费

一．常用缓冲溶液的配制方法1．甘氨酸—盐酸缓冲液（0.05mol/L）xml0.2mol/L甘氨酸＋yml0.2mol/LHCl，再加水稀释至200mlpHx/mly/mlpHx/mly/ml2.25044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸Mr=75.07，0.2mol/L甘氨酸溶液含15.01g/L2．甘氨酸—氢氧化钠缓冲液（0.05mol/L）xml0.2mol/L甘氨酸＋yml0.2mol/LNaOH，加水稀释至200mlpHx/mly/mlpHx/mly/ml8.6504.09.6508.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.810.65045.5甘氨酸Mr=75.07，0.2mol/L甘氨酸溶液为15.01g/L3．邻苯二甲酸—盐酸缓冲液（0.05mol/L，20℃）xml0.2mol/L邻苯二甲酸氢钾＋yml0.2mol/LHCl，再加水稀释到20mlpHx/mly/mlpHx/mly/ml2.254.6703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.032邻苯二甲酸氢钾Mr=204.23，0.2mol/L邻苯二甲酸氢钾溶液含40.85g/L4．柠檬酸—氢氧化钠—盐酸缓冲液pHNa＋浓度/mol/L柠檬酸/gC6H8O7·H2O氢氧化钠/gNaOH(97%)盐酸/mlHCl(浓)最终体积/L*12.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610使用时可以每升中加入1g酚，若最后pH值有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，置冰箱保存。5．磷酸氢二钠—柠檬酸缓冲液pH0.2ol/LNa2HPO4/ml0.1mol/L柠檬酸/mlpH0.2mol/LNa2HPO4/ml0.1mol/L柠檬酸/ml2.20.4019.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55Na2HPO4Mr=141.98，0.2mol/L溶液为28.40g/L，Na2HPO4·2H2OMr=178.05，0.2mol/L溶液为35.61g/LC6H8O7·H2OMr=210.14，0.1mol/L溶液为21.01g/L26．柠檬酸—柠檬酸钠缓冲液（0.1mol/L）pH0.1mol/L柠檬酸/ml0.1mol/L柠檬酸钠/mlpH0.1mol/L柠檬酸/ml0.1mol/L柠檬酸钠/ml3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.210.8柠檬酸C6H8O7·H2O，Mr=210.14，0.1mol/L溶液为21.01g/L柠檬酸钠Na3C6H5O7·2H2O，Mr=294.12，0.1mol/L溶液为29.41g/L7．乙酸—乙酸钠缓冲液（0.2mol/L，18℃）pH0.2mol/LNaAc/ml0.2mol/LHAc/mlpH0.2mol/LNaAc/ml0.2mol/LHAc/ml3.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.60NaAc·3H2O，Mr=136.09，0.2mol/L溶液为27.22g/L8．邻苯二甲酸氢钾—氢氧化钠缓冲液50ml0.1mol/L邻苯二甲酸氢钾＋xml0.1mol/L氢氧化钠，加水稀释到100mlpHx/mlpHx/mlpHx/ml4.11.34.816.55.536.64.23.04.919.45.638.834.34.75.022.65.740.64.46.65.125.55.842.34.58.75.228.85.943.74.611.15.331.64.713.65.434.1邻苯二甲酸氢钾Mr=204.23，0.1mol/L溶液为20.42g/L9．磷酸盐缓冲液(1)磷酸氢二钠—磷酸二氢钠缓冲液（0.2mol/L）pH0.2mol/LNa2HPO4/ml0.2mol/LNaH2PO4/mlpH0.2mol/LNa2HPO4/ml0.2mol/LNaH2PO4/ml5.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.051.08.094.75.36.955.045.0Na2HPO4·2H2O，Mr=178.05，0.2mol/L溶液为35.61g/LNa2HPO4·12H2O，Mr=358.22，0.2mol/L溶液为71.64g/LNaH2PO4·H2O，Mr=138.01，0.2mol/L溶液为27.6g/LNaH2PO4·2H2O，Mr=156.03，0.2mol/L溶液为31.21g/L(2)磷酸氢二钠—磷酸二氢钾缓冲液（1/15mol/L）pH1/15mol/L1/15mol/LpH1/15mol/L1/15mol/L4Na2HPO4/mlKH2PO4/mlNa2H...