精品文档---下载后可任意编辑Btbd7 基因在 SD 大鼠牙根发育早期的讨论中期报告Introduction:Btbd7 gene mutations have been linked to tooth agenesis in humans. In this study, we examined the expression pattern of Btbd7 during early tooth development in the Sprague-Dawley (SD) rat.Methods:Rat embryos at various developmental stages were collected and the mandibles were dissected for RNA extraction and quantitative real-time PCR analysis. In situ hybridization was used to detect the spatial and temporal expression pattern of Btbd7 in mandibular first molar tooth germs. Immunofluorescence analysis was performed to detect the cellular localization of Btbd7 protein.Results:Our results showed that Btbd7 mRNA expression was detected in the mandibular first molar tooth germs from embryonic day 14 (E14) to postnatal day 7 (P7). The peak of expression was observed at E18 and P1. In situ hybridization and immunofluorescence analysis revealed that Btbd7 was primarily expressed in dental papilla mesenchyme cells, including the preodontoblasts and odontoblasts. Btbd7 protein was detected in the cytoplasm and nucleus of these cells.Conclusions:Our study suggests that Btbd7 is expressed in dental papilla mesenchyme cells during early tooth development in SD rat and may play a role in tooth morphogenesis. Further studies are needed to investigate the precise function of Btbd7 in tooth development.
