精品文档---下载后可任意编辑CD28B7 信号在再生障碍性贫血 T 淋巴细胞异常活化中的作用的开题报告Introduction:Refractory Anemia (RA) is a type of myelodysplastic syndrome (MDS) which is characterized by inadequate production of mature blood cells leading to anemia. Abnormal activation of T lymphocytes has been proposed as one of the mechanisms of RA. T lymphocytes are activated by interaction between T-cell-specific antigen receptor (TCR) and antigen presented by major histocompatibility complex (MHC) on antigen-presenting cells (APC). Costimulatory receptors like CD28 on T cells and CD80/CD86 on APC provide secondary activation signals required for the activation of T cells. The CD28-CD80/CD86 interaction has been implicated in the pathogenesis of various autoimmune diseases. In this study, we aim to investigate the role of CD28-CD80/CD86 signaling in the abnormal activation of T lymphocytes in RA.Hypothesis:CD28-CD80/CD86 signaling contributes to abnormal activation of T lymphocytes in RA, leading to the production of inflammatory cytokines and ultimately causing bone marrow failure.Objectives:1. To assess the expression of CD28 and its ligands (CD80/CD86) on T cells and APCs, respectively, in RA patients.2. To evaluate the effect of CD28-CD80/CD86 signaling on T-cell activation and the production of inflammatory cytokines in RA patients.3. To investigate the mechanism by which CD28-CD80/CD86 signaling leads to abnormal T-cell activation in RA patients.Methodology:1. Patients: We will include 20 RA patients and 20 healthy controls. The RA patients will be diagnosed based on the World Health Organization criteria.精品文档---下载后可任意编辑2. Isolation of T cells and APCs: CD4+ T cells will be isolated from peripheral blood mononuclear...
