精品文档---下载后可任意编辑DDT 降解质粒 pDOD 及其对土壤中残留 DDTs 降解的生物强化的开题报告摘要：残留 DDTs（有机氯农药）是一种常见的污染物，具有毒性和对生态环境的潜在威胁。生物降解是一种有效的处理和治理残留 DDTs 的方法之一。本文设计了一种 DDT 降解质粒 pDOD，并通过构建转化菌株EA08-DOD 来实现生物强化对土壤中残留 DDTs 的降解。首先，在 pBBR1MCS-5 质粒上插入 T4 基因编码的四个蛋白质序列，构建出 DDT 降解质粒 pDOD。然后，将此质粒转化到 EA08 菌株中，构建出具有 DDT 降解能力的转化菌株 EA08-DOD。最后，在实验室模拟土壤环境中，使用 EA08-DOD 菌株对含有 DDTs 的土壤进行生物处理。经过 48 小时的生物强化处理，残留 DDTs 的降解率可达到 80%以上。本讨论结果表明，DDT 降解质粒 pDOD 和转化菌株 EA08-DOD 对残留 DDTs 的降解具有良好的处理效果，生物强化处理可以成为一种有效的、环境友好的 DDTs 污染物治理方法。关键词：DDTs，生物降解，转化菌株，生物强化，污染物治理。Abstract:Residual DDTs (organochlorine pesticides) are common pollutants that have toxicity and potential threats to the ecological environment. Biodegradation is an effective method for treating and controlling residual DDTs. In this paper, a DDT-degrading plasmid pDOD was designed, and a transformation strain EA08-DOD was constructed to achieve biological enhancement of the degradation of residual DDTs in soil.First, four protein sequences encoded by T4 genes were inserted into the pBBR1MCS-5 plasmid to construct the DDT-degrading plasmid pDOD. Then, this plasmid was transformed into the EA08 strain to construct a transformation strain EA08-DOD with DDT-degrading ability. Finally, in the laboratory simulated soil environment, EA08-DOD strain was used for biotreatment of soil containing DDTs. After 48 hours of biological enhancement treatment, the degradation rate of residual DDTs can reach more than 80%.The results of this study show that the DDT-degrading plasmid pDOD and the transformation strain EA08-DOD have 精品文档---下载后可任意编辑good treatment effect on the degradation of residual DDTs, and biological enhancement treatment can become an effective and environmentally friendly method for the treatment of DDTs pollutants.Keywords: DDTs, biodegradation, transformation strain, biological enhancement, pollution control.
