精品文档---下载后可任意编辑鳜肌肉生长抑制素、肌酸激酶基因的克隆与组织表达的开题报告【摘要】本文主要介绍了鳜（Megalobrama amblycephala）肌肉生长抑制素（MSTN）和肌酸激酶（CK）基因的克隆和组织表达讨论。采纳PCR 技术从鳜肝脏组织中克隆得到了 MSTN 和 CK 基因，经测序验证，序列长度分别为 1.49 kb 和 2.20 kb，具有典型的成熟肽片段和保守结构域。同时，利用 qRT-PCR 技术对鳜不同组织中的 MSTN 和 CK 基因表达水平进行了分析。结果表明，MSTN 基因主要在肌肉和心脏中高表达，CK 基因则在肌肉中表达最为明显。本讨论结果为深化了解鳜肌肉生长调控机制提供了重要基础。【关键词】鳜肌肉生长抑制素，肌酸激酶，基因克隆，组织表达，qRT-PCR【Abstract】This paper mainly introduces the cloning and tissue expression of myostatin (MSTN) and creatine kinase (CK) genes in Megalobrama amblycephala. The MSTN and CK genes were cloned by PCR from the liver tissue of M. amblycephala, and the sequences were confirmed by sequencing. The lengths of the sequences were 1.49 kb and 2.20 kb, respectively, and both genes had typical mature peptide segments and conserved domains. Meanwhile, the expression levels of the MSTN and CK genes in different tissues of M. amblycephala were analyzed by qRT-PCR. The results showed that the MSTN gene was mainly expressed in muscle and heart, while the CK gene was most prominently expressed in muscle. The results of this study provide an important basis for understanding the regulation mechanism of muscle growth in M. amblycephala.【Keywords】Megalobrama amblycephala, myostatin, creatine kinase, gene cloning, tissue expression, qRT-PCR
