时间分辨免疫荧光与化学发光法、酶联免疫法测定乙肝标志物的比较摘 要 目的: 比较化学发光免疫测定(CL)、酶联免疫(EIA)与时间分辨免疫荧光(TRFIA)三种方法检测乙肝病毒免疫标志物的优缺点。方法: 用上述三种方法的检测试剂及仪器对 189 例临床血清标本分别进行检测,然后进行结果分析。结果: 三者对乙肝表面抗原的检测灵敏度均达到了 0.1ng/ml。对 HBcAb 的测定,以 CL 的灵敏度最高,ELISA 最低。对表面抗体、E 抗原及 E 抗体的检测,相互符合率均在 95%以上,但对核心抗体的检测符合率, TRFIA 与 CL 为 83.23%,TRFIA 与 EIA 为85.19%。结论: 三种方法的检测性能相差不大,但操作性各有特点,应按各实验室具体情况加以选择。 关键词:化学发光法,酶联免疫试验,时间分辨荧光免疫测定 本文作者:肖征 周薇薇 白立彦 赵莉萍 Comparison of chemiluminescence, ELISA and time-resolved fluoroimmunoassay in detecting Hepatitis B markers 肖征,副主任医师,副教授周薇薇,主管技师白立彦,主管技师赵莉萍,主管技师解放军总医院微生物科,100853 北京Zheng Xiao, Weiwei Zhou, Liyan Bai, Liping ZhaoGeneral Hospital of PLA, Beijing, 100853 PRC AbstractSubject: To compare three deferent assays to see their abilities in detecting hepatitis B markers. Methods : By using Chemiluminescence (CL), ELISA and Time-resolved fluoroimmunoassay (TRFIA), 189 serum samples were tested for hepatitis B markers, then the results were analyzed. Results: The three assays all can detect as low as 0.1ng/ml of HBsAg. But in HBcAb, The CL showed the highest sensitivity, ELISA showed the lowest sensitivity. The coincidences of the three methods in detecting HBsAb, HBeAg, and HBeAb are normally above 95%, but for HBcAb, the coincidence was 83.23% between TRIF and CL, and 85.19% between TRIFA and EIA. Conclusion: There were not much difference among these three methods regarding their abilities in detecting hepatitis B markers, but the maneuver flexibilities of each method differed...