不同材料制备大鼠神经病理性疼痛CCI模型的比较VIP免费

Observationofstabilityofasimpletype2diabeticratmode:lcombinationofhigh�fatdiet�fedandlow�dosestreptozotocin�induceddiabeticratLIANGHai�xia1,2,YUANHai�yan2,LIHuan�de1,2,ZHANGSong3,XIANGDa�xiong1,2(1.SchoolofPharmaceuticalSciencesofCentralSouthUniversity,Changsha�410013,China;2.TheClinicalPharmacy&PharmacologyResearchInstitute,3.DeptofEndocrinology,SecondXiangyaHospitalofCentralSouthUniversity,Changsha�410011,China)Abstract:Aim�Todevelopasimpleratmodeloftype2diabe�testhatwassuitableforlong�termpharmacologicalstudies.Methods��Type2diabeteswasinducedbycombinationofhigh�fatdiet�fedandlow�dosestreptozotocin(STZ,45mgkg-1,ip)injectioninSDrats,thentheconcentrationsofbloodserumfastedbloodglucose(FBG),triglycerides(TG),totalcholesterol(CHO)andinsulin(INS)weredetermined72haf�terSTZinjection,theinsulinsensitivityindex(ISI)wascalcu�latedwithHOMAmethod,ratswithaFBGlevelabove10�0mmolL-1andISIbelowthemeanlevelofnormalcontrolgroup!swereconsidereddiabetic;meanwhile,non�fastingbloodglucose(NFBG)wasmeasuredweeklyandchangesofbodyweight,foodandwaterintakewerealsomonitoredregularly;∀5weeksafterSTZinjection,theresponseofthismodeltophen�formin(75mgkg-1)wastestedbyoraladministrationfor3daystodeterminethesuitabilityandvalidityofthisratmodelforlong�termpharmaceuticaltesting.Results��Ratsfedthefat�enricheddietfor4weeksweighed30gmorethanthenormalchow�fedcontrolones,buttherewasnosignificantdifferencebetweenthetwogroups;meanwhile,foodandwaterintakeofthetwogroupswassimilar.72hafterSTZinjection,thesuccessrateoftype2diabeticanimalmodelreached91�7%.∀Duringtheexperimentalperiodoflong�termstabilityobservation,thosetype2diabeticratsalwayshadhigherNFBG(>20�0mmolL-1).#Oraladministrationofphenforminfor3dayssignificantlyre�ducedNFBGofthosetype2diabeticrats.Conclusions�Estab�lishmentofthistype2diabeticratmodelwassuccessfulandsimple;besides,thisdiabeticmodelwasstableandsuitableforlong�termpharmacologicalstudies.Keywords:type2diabetesmellitus;insulinresistance;animalmode;lstreptozotocin不同材料制备大鼠神经病理性疼痛CCI模型的比较马�骋,李翠贤,易建良,闫丽萍(南京中医药大学江苏省针药结合实验室,江苏南京�210029)中国图书分类号:R�331;R�332;R322�85;R363�332;R745文献标识码:A�文章编号:1001-1978(2008)04-0555-03摘要:目的�比较肠线、丝线、PE套管3种材料制备的CCI模型的造模效果。方法�用肠线、丝线4道轻结扎大鼠坐骨神经干,或用PE90套管造成大鼠坐骨神经干慢性压迫性损伤,测定伤足底热痛阈和机械痛阈评定模型的效果。结果�3种材料造模后d9和d15测定热痛阈和机械痛阈,比造模前均下降,其中肠线CCI模型对热痛阈的影响强于丝线或PE管CCI模型(d9,P<0�05;d15,P<0�01)。3种材料制备的CCI模型间机械痛阈差异无显著性。结论�3种材料均可造成满意的CCI模型,但肠线造模的效果优于丝线和PE管。收稿日期:2007-11-21,修回日期:2008-01-16基金项目:江苏省自然科学基金资助项目(NoBK2006239);江苏省高校自然科学重大基础研究资助项目(No05KJA36011)作者简介:马�骋(1954-),男,高级实验师,研究方向:神经生理学,Te:l025�86798142,E�mai:lcma@njutcm.edu.cn;闫丽萍(1960-),女,博士,副研究员,研究方向:神经生物学,Te:l025�86798142,E�mai:lht990@126.com关键词:坐骨神经慢性压迫模型(CCI);铬制羊肠线;丝线;PE90套管;大鼠��1988年Bennet等用铬制羊肠线建立了坐骨神经慢性压迫模型(chronicconstrictioninjuryofthesciaticnervemode,lCCI)[1],这是首个可以利用检测机械和热痛阈来评价痛相关行为的模型,具有外周和中枢敏化特征。此模型与临床神经病理性疼痛特征有相似之处,因此,在与疼痛学有关的研究中...