毕业论文HPLC法测定不同加工方法的半夏中鸟苷总结VIP免费

本科毕业论文题目HPLC法测定不同加工方法的半夏中鸟苷的含量学院理学院专业应用化学毕业届别2015届姓名指导教师职称教授甘肃农业大学教务处制二〇一五年三月普胜：HPLC法测定不同加工方法的半夏中鸟苷的含量目录摘要.....................................................................1关键词...................................................................1Abstract...................................................................1Keywords..................................................错误！未定义书签。前言.....................................................................11材料....................................................................21.1原生半夏..............................................................21.2硫磺熏蒸的半夏........................................................21.3焦亚硫酸钠处理的半夏..................................................21.4仪器和试剂............................................................22实验方法................................................................22.1半夏药品供试液的制备..................................................22.2鸟苷对照品溶液的制备..................................................22.3线性关系考察..........................................................42.4精密度实验............................................................52.5重复性实验............................................................52.6样品含量测定..........................................................63.......................................................................64结论...................................................................6参考文献.................................................................6致谢.....................................................................8普胜：HPLC法测定不同加工方法的半夏中鸟苷的含量1HPLC法测定不同加工方法的半夏中鸟苷的含量普胜（甘肃农业大学理学院应用化学专业，甘肃兰州，730070）摘要：目的：建立半夏中鸟苷的含量测定方法。方法：采用高效液相色谱法．色谱柱为AgilentZorbaxSB-C18(4.6×250mm，5um)．流动相为甲醇：0.01％醋酸(3∶97)；检测波长254nm；流速为1.0mL·min-1。结果:本法精密度高．鸟苷溶液在0.01042～0.09378mg·ml1的浓度范围内具有良好的线性关系(r=0.9998)。结论：本方法简便，准确．重现性好，可作为半夏的含量测定方法。关键词：半夏；鸟苷；高效液相色谱法DeterminationoftheGuanosineinPinelliaternata(Thumb.)Breit.FromVariedProcessingMethodsbyHPLCPUSheng（MajorinAppliedChemistryintheCollegeofscienceofGansuAgricultureUniversity，GansuLanzhou，730070）Abstract：Objective：toestablishamethodfordeterminationoftheGuanosineinPinelliaternateBreit．Methods：UsingaHPLCmethodwithAgilentZorbaxSB_Cl8(4.6X250mm，5um)column；themobilephasewasmethanol0.01%ethanoicacid(3:97)；thedetectionwavelengthwassetat254nm；flowratewas1.0mL·min-1；Theprecisionandstabilitywerefinehhelineanrangewithin0.01042～0.09378mg·mL-1,r=0.9998.Conclusion:ThemethodWassimple，accurateandreproducible．Andcan_beusedtodeterminetheGuanosineinPinelliaternateBreit.Keywords：Pinelliaternata(Thumb.)Breit.；theGuanosine；HPLC前言半夏为天南星科植物半夏Pinelliaternata(Thunb．)Breit的块茎，首载于《神农本草经》，性温，味辛，有小毒，归脾、胃、肺经。具有燥湿化痰，降逆止呕，消痞散结的功效[1]。半夏含有生物碱、β-谷甾醇、多糖、氨基酸、挥发油、半夏蛋白及无机元素等多种成分。半夏水溶性化学成分鸟苷通常作为半夏质量的评价指标，通常采用反相高效液相色谱进行分析检测[2-...