0.5mol/L Tris(pH6.8)10% Aps共10% Aps25303uddH2O1.50.62.TEMED4% 浓缩胶一块胶30%Bis(丙烯酰胺)0.3310% SDS2.10% SDS100101.5mol/L Tris(pH8.8)6% 分离胶ddH2O5.30%Bis(丙烯酰胺)2.0TEMED8u共10% Aps共10% Aps7% 分离胶ddH2O4.830%Bis(丙烯酰胺)2.32块胶1010TEMED8u2块胶101.5mol/L Tris(pH8.8)2.10% SDS1001.5mol/L Tris-HCl2.10% SDS1007.5% 分离胶ddH2O4.830%Bis(丙烯酰胺)2.550-TEMED5-8共10% Aps共10% Aps9% 分离胶ddH2O4.30%Bis(丙烯酰胺)3.22块胶1010TEMED6u2块胶101.5mol/L Tris-HCl2.10% SDS1001.5mol/L Tris-HCl2.10% SDS10010% 分离胶ddH2O4.30%Bis(丙烯酰胺)3.310TEMED4u共10% Aps共10% Aps12% 分离胶ddH2O3.30%Bis(丙烯酰胺)4.02块胶1010TEMED4u2块胶101.5mol/L Tris(pH8.0)2.10% SDS10030%Bis(丙烯酰胺)5.01.5mol/L Tris(pH8.8)2.※分离蛋白线性有效范围：12-60kDa15% 分离胶ddH2O2.10% SDS5050共2块胶10※分离蛋白线性有效范围：10-43kDaTEMED2u25ul30ul3ul1.53ml0.625ml2.5ml0.3325ml2.6ml100ul100ul5.2ml2.0ml8ul4.82ml2.38ml10ml100ul8ul10ml2.6ml100ul2.5ml100ul胶4.85ml2.5ml50-80ul5-8ul4.1ml3.2ml10ml100ul6ul10ml2.5ml100ul2.5ml100ul胶4.0ml3.3ml100ul4ul胶3.2ml4.0ml10ml100ul4ul10ml2.6ml100ul5.0ml2.6ml12-60kDa胶2.2ml50ul50ul10ml10-43kDa2ul
